Bio-Helix

Blueray prestained protein standard pm006 0500

彩色標記蛋白質電泳標準品 / BlueRAY Prestained Protein Ladder(10 to 180 kDa)

型號: PM006-0500
規格: 500 μl
產地: 台灣
報價: 2,450 元
運費: 以PDF報價單為準
交期: 期貨,依供應商說明
供應商: 良知股份有限公司
聯絡電話: 02-26518190

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詳細 資訊

The BlueRAY Prestained Protein Ladder is a three-color protein standard with 10 pre-stained proteins covering a wide range molecular weights for 10 to 180 kDa. Proteins are covalently coupled with a blue chromophore except for two reference bands (one green and one red band at 25 kDa and 75 kDa respectively) when separated on SDS-PAGE (Tris-glycine buffer). The BlueRAY Prestained Protein Ladder is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins. The ladder is supplied in gel loading buffer and is ready to use.

 

-Guide for Molecular Weight Estimation (kDa)

Migration patterns of BlueRAY Prestained Protein Ladder in different electrophoresis conditions are listed below :

• 3 μl or 5 μl per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel, respectively.
• 2~3 μl per well for general Western transferring.
• Apply more for thicker (>1.5 mm) or larger gel.

 

Contents : 
Approximately 0.2~0.4 mg/ml of each protein in buffer (20 mM Trisphosphate pH 7.5 at 25°C), 2% SDS, 0.2 mM Dithiothreitol, 3.6 M Urea, and 15% (v/v) Glycerol.

Quality Control : 
5 μl of BlueRAY Prestained Protein Ladder (PM006-0500) resolves 10 bands in 4-20% SDS-PAGE (Tris-glycine buffer) and after Western blotting to PVDF membrane.

Storage : 
Stable for up to 2 weeks at 25°C.
Stable for up to 3 months at 4°C.
Stable for up to 24 months at -20°C.